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Confocal Imaging and Analysis

AT Ashley L Titan
MD Michael Davitt
DF Deshka Foster
AS Ankit Salhotra
SM Siddharth Menon
KC Kellen Chen
EF Evan Fahy
ML Michael Lopez
RJ R Ellen Jones
IB Ioana Baiu
AB Austin Burcham
MJ Michael Januszyk
GG Geoffrey Gurtner
PF Paige Fox
CC Charles Chan
NQ Natalina Quarto
ML Michael Longaker
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The cryopreserved 8-μm sections on Superfrost Plus microscope slides (Fisher Scientific) as well as the chambered cell culture slides were imaged using laser scanning confocal microscopy via the Leica WLL TCS SP8 Confocal Laser Scanning Microscope (Leica Microsystems) located in the Cell Sciences Imaging Facility (Stanford University, Stanford, CA). The 10× objectives were used (10× HC PL APO, air, N.A. 0.40). Image analysis was performed using Fiji software (ImageJ, NIH). Photoshop was utilized for image intensity analysis using the histogram tool.

Copyright and License information:  ©2022 The Author(s) 2022. Published by Oxford University Press.
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