Analysis of Tetracycline Degradation Products by HPLC

SW Shaochen Wang
XG Xia Gao
YG Yuejiao Gao
YL Yanqing Li
MC Mingming Cao
ZX Zhenhua Xi
LZ Lixing Zhao
ZF Zhiyang Feng
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Products generated from enzymatic inactivation of tetracycline were analyzed by reverse phase HPLC (InertSustain® C18, 4 × 250 mm, 5 μm) using a linear gradient of H2O:MeOH (20:80 to 80:20) over 20 min at 1 ml/min.

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