2.10. Acute slice preparation

Male C57BL/6 mice, aged P28–35, were anesthetized with isoflurane. After decapitation, brains were dissected quickly and placed in ice‐cold NMDG cutting solution containing (in mM) 92 NMDG, 82 NaCl, 2.5 KCl, 1.2 NaH₂PO₄, 20 HEPES, 30 NaHCO₃, 25 Glucose, 5 Na‐ascorbate, 3 Na‐pyruvate, 2 Thicurea, 10 MgSO₄, 0.5 CaCl₂, and pH 7.3–7.4 (300–310 mOsM). Slices (250 μm) were collected with a vibratome (Leica Instruments) and maintained in continuously oxygenated artificial cerebrospinal fluid (ACSF) for at least 30 min at 34°C and then at room temperature for 30 min before recording. Individual slices were transferred to a submerged recording chamber visualized with infrared optics microscope (Nikon), where they were perfused continuously with carbogen‐buffered ACSF.