The antibacterial properties of H. aspersa Müller slime have been determined against P. aeruginosa have been isolated and identified at Plant, Animal and Agro-Industry Productions Laboratory (PAAP lab), Ibn Tofail University, and stored at −4 °C in glycol. Other reference strains are used in this study (L. monocytogenes (ATCC 7644), S. aureus (ATCC 29213), and E. coli (ATCC 35218)). In order to obtain live and cultivable bacteria, 1 mL of stored bacterial suspension was mixed with 2 mL of nutrient broth.
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