MTT cell viability assay

Cells, inoculated in 96-well plates (2×10⁴ cells/well), were treated with the test molecule for 48 hours while the negative control received only growth medium supplemented with DMSO (< 0.2%) used as a vehicle. The clinical anticancer drug cisplatin was used as a positive control. Following incubation, all cells received MTT reagent (10 μL) including controls, and the plate was incubated at 37 °C and 5% CO₂ for 2 hours. Colorimetric quantification was performed at an optical density of 570 nm. The absorbance of the sample wells was corrected by subtracting that of the blank wells. The percentage of cell viability was calculated and normalized to the controls.