2.2.4. Histological staining

Cryosections of vastus lateralis muscle samples (7 μm) were co‐stained for lipid (Bodipy 493/503–green) and for anti‐human myosin heavy chain (hMYH)7 to stain type I fibers largely as described previously. ³² Briefly, after fixation in formalin, sections were washed 3 times in PBS and blocked using 10% goat serum. After washing with PBS, they were incubated with anti‐hMYH7, followed by dye‐labeled secondary antibodies (red) to visualize the type I fibers. They were further stained with Bodipy 493/503 (green) in the dark. They were then washed in PBS, dried, covered with Prolong Gold™, and mounted.