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Splinkerette PCR for sequencing of transposon integration sites
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Genomic DNA was isolated and digested with Sau3AI. The digested genomic DNA was ligated to 50 nM of splinkerette adaptors using T4 ligase and ligation buffer from NEB. Nested PCR was used to amplify the genomic DNA sequence adjacent to the 5′ and 3′ TIR ends. The PCR products were sequenced to determine the precise transposition sites.
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