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Measurement of Prostaglandin E2 (PGE2) Generation
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Cells (1 × 106 cells/ml) in a 24-well plate were pre-incubated with three lipids or 1% DMSO. Culture supernatants were collected at 24 h after incubation to determine PGE2 levels using ELISA kits (Enzo Life Sciences, USA) according to the manufacturer’s instructions.
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