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Quantitation of Total Bacterial Load in Wounds
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Tissue samples were obtained from each wound after cleansing for debris and/or surface contamination, as previously described [22, 28]. Tissue samples were analyzed by a CLIA-certified laboratory (Eurofins Central Laboratory, Lancaster, PA) for bacterial load and species using aseptic, gold-standard techniques. Quantitative culture was performed as previously described [22], where microbiologists were blinded to the results of CSS assessment and FL-imaging.
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