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2.4.3. Contextual fear conditioning test (CFC test)

AK Ayako Kawaminami
DY Daisuke Yamada
TY Toshinori Yoshioka
AH Azumi Hatakeyama
MN Moeno Nishida
KK Keita Kajino
TS Tsuyoshi Saitoh
HN Hiroshi Nagase
AS Akiyoshi Saitoh
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On the conditioning day (Day 1), mice received eight 0.8‐mA foot shocks (1 s in duration at 30 s intervals) by shock generator (ENV‐414; Med Associates) in the conditioning chamber (20 cm × 20 cm × 33 cm; LabDesign). After a 24‐h later (Day 2), mice were placed back into the same chamber without foot‐shock for 6 min. Freezing behavior was manually analyzed based on previous reports, 12 during each session, by training the experimenter.

Copyright and License information:  ©2023 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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