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Harvested cells were lysed using the cell lysis buffer, centrifuged at 12,000 × g for 10 min, and the supernatant was collected. Protein content of the lysate was quantified using the Pierce BCA Protein Assay Kit. Then, 40 μL of the supernatant (30 μg) and 100 μL of L-DOPA (10 mM) were mixed in a 96-well plate and incubated at 37 °C for 2 h. Cellular tyrosinase activity was assayed by measuring L-DOPA metabolite absorbance at 475 nm.
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