Inhibition of FAS by C75 treatment

Unless otherwise stated, Wolbachia-free and wMel-Aag2 lines were seeded and infected as described above. Prior to DENV-2 infection cells were pre-treated with 1µM fatty acid synthase (FAS) inhibitor C75 (Abcam) or DMSO diluent only for 24 h. Cells were mock-infected or DENV-2-infected at MOI 1 for 24 h, and C75 was maintained during the time of infection in Aag2 cell culture medium with 10% FBS. LD quantification, viral replication, and TEM imaging were performed as described above.