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Effector T cells (CD4+CD25–) were obtained from WT mice by magnetic separation and subsequently labeled with 5 nM CFSE (Invitrogen) for 10 minutes at 37°C. CFSE-labeled effector T cells (1 × 105) were then cocultured with Tregs (CD4+CD25+) according to the indicated ratios in the presence of anti-CD3 (clone: 145-2C11;BD) and anti-CD28 (clone: 37.51; BD). After 72 hours, the cells were harvested to measure CFSE dilution by flow cytometry.
Copyright and License information: ©2023 Cao et al.
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