2.16. Patient‐derived xenograft (PDX) mouse models

For generation of PDX models, ²² freshly resected tumor tissues were obtained from two patients with ARID1A‐mutated lung cancer and minced to ~2 mm³ tissue fragments. The two lung cancer patients did not receive any anticancer treatment before surgery. The tissue fragments were implanted subcutaneously in recipient male NOD‐SCID‐IL2RgammaC‐null (NSG) mice (5 weeks old). When the xenograft tumors reached approximately 200 mm³, the mice were randomly divided into four groups: the control group was injected with phosphate‐buffered saline (PBS), the anti‐P4HB group was injected intraperitoneally with anti‐P4HB (100 μg; once every 4 days for 16 days), the cisplatin group was injected intraperitoneally with cisplatin (5 mg/kg; twice per week for 2 weeks), and the anti‐P4HB + cisplatin group was injected intraperitoneally with anti‐P4HB (100 μg; once every 4 days for 16 days) and cisplatin (5 mg/kg; twice per week for 2 weeks). Each group had five mice. The tumor volume was measured by caliper, and tumor growth curves were compared.