Heatmap and hierarchical clustering

An unsupervised hierarchical clustering of the 1,167 introns with altered splicing efficiency (ΔSI ≥ 0.05, FDR adjust p.value <0.05) in our HEK.dDHX15 experiments and the G-patch factor KD experiments was performed using ComplexHeatmap v2.2.0,⁶⁹ with (1 – Pearson’s correlation) as the clustering distance. Upset plot of the intersection size of introns with altered splicing were also performed using make_comb_mat function with ComplexHeatmap. Correlation heatmap generated using corrplot v0.92.