5.4. Southern blot hybridization

BK Betim Karahoda
BP Brandon T. Pfannenstiel
ÖS Özlem Sarikaya-Bayram
ZD Zhiqiang Dong
KW Koon Ho Wong
AF Alastair B. Fleming
NK Nancy P. Keller
ÖB Özgür Bayram
ask Ask a question
Favorite

Southern blot hybridization experiments were carried out using Roche DIG Nucleic Acid Detection Kit (Roche, 11175041910). Amplification of 5′UTR region (each yielding approximately 1.2 kbp) probes were carried out using non-radioactive digoxigenic (DIG) labelling kit (Roche, 11093657910).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A