Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Pediatric samples

CB Cristina Bugarin
LA Laura Antolini
CB Chiara Buracchi
SM Sergio Matarraz
TC Tiziana Angela Coliva
VV Vincent H. van der Velden
TS Tomasz Szczepanski
EC Elaine Sobral da Costa
AS Alita van der Sluijs
MN Michaela Novakova
EM Ester Mejstrikova
SN Stefan Nierkens
FM Fabiana Vieira de Mello
PF Paula Fernandez
CA Carmen Aanei
ŁS Łukasz Sędek
LS Luisa Strocchio
RM Riccardo Masetti
LS Laura Sainati
JP Jan Philippé
MV Maria Grazia Valsecchi
FL Franco Locatelli
JD Jacques J.M. van Dongen
AB Andrea Biondi
AO Alberto Orfao
GG Giuseppe Gaipa
ask Ask a question
Favorite

Samples for flow cytometric immunophenotyping were collected in eight EuroFlow centers (Brazil, Czech Republic, France, Italy, the Netherlands, Poland, Spain and Switzerland) from 31 children newly diagnosed as JMML according to the WHO 2016 criteria.4 From each child either BM (n=22) or PB (n=9) was collected. In eight of 31 JMML patients paired BM/ PB samples were available, obtaining a total of 39 samples (22 BM and 17 PB) for the analyses.

The immunophenotype of JMML BM/PB cells was compared with that of BM cells from 29 children without hematological malignancies (referred in the following as control group) or with that of BM/PB cells from nine children presenting with a suspected diagnosis of JMML which was not subsequently confirmed (referred in the following as non-confirmed JMML patients).

For validation purposes, an additional cohort consisting of four control subjects and three JMML patients was collected.

Informed consent was obtained from each patient and/or his/her legal guardian.

Copyright and License information:  ©2024 Ferrata Storti Foundation
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A