Laser capture microdissection

To prepare for laser capture microdissection (LCM), iPSC-iNs were plated onto membrane slides coated with poly-ornithine (100 μg ml⁻¹, Sigma Aldrich) and laminin (100 μg ml⁻¹, Invitrogen) in neuron maturation medium and cultured until matured. Following neuronal maturation, slides were taken for LCM. Maturation medium was carefully removed from the slides without detaching the neurons and LCM was immediately conducted. Neurites were identified at 40× magnification, marked using drawing tools, and laser capture was performed on these areas onto CapSure LCM macro caps. Laser energy was in the capture method (810 nm).