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HEK293 cells with MxA KO or overexpression, or control cells, were incubated with DBV (0.1 MOI) at 37°C for 2 h. After three washes, the cells were incubated at 37°C for the indicated time. The cell culture supernatants collected at the indicated time points were subjected to viral titration by TCID50 assays using Vero cells as previously described (16, 18, 74, 75).
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