2.2. Cell Lines and Culture Conditions

MCF-7 human breast cancer and human monocyte THP-1 cell lines were obtained from the American Type Culture Collection (ATCC). Human dermal FB (HDFa) cell line was purchased from ThermoFisher scientific. MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco), 1% l-glutamine, 2.7% sodium bicarbonate, 1% HEPES buffer, and 1% penicillin/streptomycin solution (GPS, Sigma). FBs were cultured in Human Fibroblast Expansion medium (ThermoFisher scientific). THP-1 cells were cultured in the Roswell Park Memorial Institute (RPMI) culture media. Cells were grown at 37 °C in an atmosphere containing 5% CO₂ and 95% humidity. Cell lines were tested routinely for mycoplasma contamination.