Y’ element telomeric probe was obtained according to [77] with minor modifications. After standard PFGE separation, Y’ sequences within particular yeast chromosomes were detected using digoxigenin labeling, anti-digoxigenin antibody and alkaline phosphatase-based chemiluminescence [72].
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.