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Hoechst 33342 staining of live cells
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Cells are inoculated into a 6-well plate. After the cells are cultured post si-1 and si-2 intervention, the culture medium is discarded. The cells are fixed with ethanol, washed twice with PBS, and stained with Hoechst 33342 stain for 15 min. After washing twice with PBS, the cells are mounted with glycerol water solution, and observed under a fluorescence microscope. The cell nuclei appear as blue fluorescence.
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