Plasmids

Lentiviral overexpression vectors for TET3-WT, TET3-CD, and TET3-CDM were subcloned into pCDH-CMV-MCS-EF1 vector, and hairpins targeting Tet3 were subcloned at AgeI/EcoRI to pLKO.1 vector. gRNA to target Pparg promoter was cloned at AarI site to pLN-U6-gRNA. Hairpin and gRNA sequences are shown in Table S2 pMD2.G and psPAX2 were gifts from Didier Trono (Addgene plasmid # 12259, #12260; http://n2t.net/addgene:12259, 12260; RRID: Addgene_12259, 12260). pLKO.1 - TRC cloning vector⁷⁴ was a gift from David Root (Addgene plasmid # 10878; http://n2t.net/addgene:10878; RRID: Addgene_10878)