Parasite culture

AP Alena Pance
BN Bee L. Ng
KM Kioko Mwikali
MK Manousos Koutsourakis
CA Chukwuma Agu
FR Foad J. Rouhani
RM Ruddy Montandon
FL Frances Law
HP Hannes Ponstingl
JR Julian C. Rayner
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Fluorescent P. falciparum parasites were cultured in complete RPMI medium (GIBCO) at 2.5% haematocrit with O- RBCs sourced from NHSBT, Cambridge. Cultures were maintained at 37°C in malaria gas (1% O2, 3% CO2 and 96% N2).

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