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2.18. Knock down of NRF2 expression by siRNA
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The siRNA targeting NRF2 were applied to knock down expression of NRF2. The scramble siRNA (Si-NC) and si-NRF2 and obtained by Invitrogen (Carlsbad, CA, USA). After seeding into 6-well plates, macrophages were transfected with 50 nM of Si-NC or Si-NRF2 using Lipofectamine 3000 (Invitrogen). The sequence of NRF2 siRNA: (5′-ATGCAACAGGACATTGAGCAA-3′).
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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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