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Preparation of brain samples for LC–MS/MS
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Each tissue sample (typically 50–150 mg for midbrain and 10–60 mg for PFC) was transferred to a lysing tube. A ninefold mass of cold water (approximately 4 °C) was added. The tissue was homogenized using the instrument's standard soft tissue program (speed 5800 rpm, cycle 2 × 15 s, pause 30 s). A 100-µL volume of the homogenate was transferred to a 2-mL tube and treated according to the procedure for blood samples.
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