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Thermal hyperalgesia detected by plantar test [15]. Rats were placed in a plastic box, allowed to acclimate for 5 minutes, and then subjected to thermal hyperalgesia test using a footpad test algesimeter (Ugo-Basile, Comerio, Italy). When radiant heat was applied to the plantar surface of the rat’s hind paw, the time until the rat avoided the paw was measured.
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