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Multiplex immunoassay for cytokine profiling
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Cytokine levels in ascites cell and CD14+ ascites cell culture supernatants were measured using Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad, catalog no. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad) according to the manufacturer's instructions. Cytokines with >10% of datapoints outside the detection range were excluded from the analyses. Remaining values lower than the detection limit were replaced by 0.5 × lowest measured value. Results are presented as fold changes between bexmarilimab- and IgG4-treated samples.
Copyright and License information: ©2023 The Authors; Published by the American Association for Cancer Research
This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
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