Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Histopathological evaluation and image acquisition

WE Walaa A. El-Sabbagh
NF Noha A. Fadel
RE Rania M. El-Hazek
AO Ahmed H. Osman
LR Laila A. Ramadan
request Request a Protocol
ask Ask a question
Favorite

Coronary artery and aortic arch tissue specimens were fixed in 10% neutral buffered formalin. The fixed specimens were then trimmed, washed and dehydrated in ascending grades of alcohol, cleared in xylene, embedded in paraffin, sectioned at 4-6U thickness and stained by hematoxylin and eosin, according to Bancroft et al.59.

The sections were evaluated by scanning the entire tissue specimen under low-power magnification (×40) and then confirmed under higher power magnification (×200 and ×400). The severity of histopathological findings was scored as (0) absent, (1) minimal, (2) mild, (3) moderate and (4) marked change. All histopathological scoring and evaluation were carried out blindly. Images were obtained under a light microscope.

Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A