4.5. Incucyte Live-Cell Proliferation Assays

For live-cell proliferation assays, 2.5 × 10³ cells/well were seeded in 96 well/plates one day before treatment (seeding volume 100 μL/well). The seeding media was replaced with drug-containing media (or DMSO, 0.1% final) 24 h post-seeding (final volume 100 μL/well). Phase imaging was performed on an Incucyte SX5 system, with image acquisition every 12 h, starting 1 h post-drug administration.