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LNP characterization

DZ Dania Zhivaki
EG Emily A. Gosselin
DS Debrup Sengupta
HC Holly Concepcion
CA Chisom Arinze
JC Jonathan Chow
AN Anastasia Nikiforov
VK Veronica Komoroski
CM Carolyn MacFarlane
CS Caitlin Sullivan
JK Jonathan C. Kagan
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Loading of mRNA into LNPs was quantified using a RiboGreen assay (ThermoFisher) following the manufacturer’s protocol. Samples were diluted to fall within the range of the standard curve. LNPs were disrupted using Triton X-100 to assess the encapsulation of mRNA into LNPs. Both total mRNA and encapsulated mRNA were quantified. The size of the LNPs was assessed using dynamic light scattering (DLS) on the NanoBrook Omni (Brookhaven). LNPs were diluted 1:10 in PBS before running on the DLS. Four 180-second measurements were recorded for each sample, with the first measurement discarded and the following three measurements used to calculate the size of the LNPs.

Copyright and License information:  ©2023 Zhivaki et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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