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Plaque assays were performed in six-well plates. Confluent HFF monolayers were infected with 50 parasites per well and treated with 500 µM indole-3-acetic acid (IAA, auxin), which prompts the degradation of mAID-tagged proteins. The viability of tet-OFF parasites was evaluated by treating infected monolayers with 1 µg/mL ATc. Plaques developed for 7 days at 37°C and then stained with crystal violet and counted. Three biological replicates of each assay were performed.
Copyright and License information: ©2023 Marsilia et al.
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