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The analysis of the LDH cytotoxicity effects of PF at various times and concentrations on gastric cancer cells was performed by an LDH assay (Thermo Scientific, Waltham, MA, USA). Gastric cancer cells (1 × 104 cells/well) were seeded and cultured in a 96-well plate containing cell culture medium. These assays were performed following the manufacturer’s instructions. The absorbance of each sample was analyzed at 490 nm using a microplate reader (Molecular Devices, CA, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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