4.8. Evaluation of Cell Viability Using a Resazurin Assay

The impact of lipid fractions on cell viability was determined using the resazurin reduction assay [63]. Macrophages were seeded at 5.0 × 10⁵ cells·well⁻¹ in a 96-well plate and allowed to stabilize in 200 μL of media overnight in the incubator. Cells were then treated with increasing concentrations of liposomes containing total extracts (TE), DGDG and sulfoquinovosyldiacylglycerol (SQDG), MGDG, and PL-and-DGTS-enriched fractions (10, 25, 50, 100, and 200 μg·mL⁻¹). After 22 h, resazurin (50 μM) was added, and cells were incubated for an additional 2 h. Absorbance was then measured at 570 and 600 nm in a Tecan infinite M200 spectrophotometer (Tecan Group, Männedorf, Switzerland). All assays were performed in biological triplicates.