2.7. Immunohistochemistry

The samples were stained with hematoxylin-eosin, followed by immunohistochemical staining. VEGF expression was measured by counting the number of new blood vessels, as VEGF markers typically stain new blood vessels brown and existing blood vessels purple color, according to the National Center for Biotechnology Information at U.S. Information (N.C.B. I). Five slides were prepared and stained for each biopsy specimen. On each slide, the total number of blood vessels was counted, and the average count for the five slides was calculated. Subsequently, the mean value for each group was determined, as presented in Table 2.

The primary antibody used in this study was the VEGF antibody. Its clone is VG1, rabbit “anti-Human” supplied by Chemi-Con Registered office: 40-42 Hatton Garden, London, England, EC1N. -An 8EB-Catalog Number: MAB3734.

We used 1 mL of rh-BMP-2 (0.25π g/mL) soluble in physiological serum (Cowell Medi Co., Ltd., Republic of Korea under the reference: BB1025) for our injections.