2.5.1. DPPH Radical Scavenging Assay

The DPPH radical scavenging assay was performed spectrophotometrically according to Tumbas Šaponjac et al. [38]. Briefly, 250 μL DPPH• solution in methanol (0.89 mM) was mixed with 10 μL of sample in a microplate well. Absorbance was measured at 515 nm after 50 min incubation in the dark at ambient temperature. Methanol was used as a blank. DPPH radical scavenging activity values were calculated using the following equation:

where A_control is the absorbance of the blank and A_sample is the absorbance of the pasta sample. The results were expressed in μmol Trolox equivalent (TE) per 100 g of pasta. Analyses were performed in three replicates.