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2.5. Live Cell Imaging and Real-Time Detection of Apoptosis
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Replicative senescent HUVECs were exposed to 0.33 µM activated simvastatin. On day two of exposure, IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent 4440 (Essen Bioscience, Ann Arbor, MI, USA), enabling real-time visualization of caspase-3/7 activation in cells, was added to the complete endothelial growth medium. Time-lapse sequences were recorded over 15 h (1 frame per 6 min) with the Olympus IX83 cellVivo live cell imaging system using both the appropriate fluorescent channel as well as bright-field imaging.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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