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2.2. Adiponectin and Interleukin-6 (IL-6) ELISAs

PM Patricia Mester
UR Ulrich Räth
SS Stephan Schmid
MM Martina Müller
CB Christa Buechler
VP Vlad Pavel
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We collected blood samples from patients within 12 to 24 h of their admission to the intensive care unit using EDTA as the anticoagulant. Plasma was separated from the blood samples. We employed the human Adiponectin DuoSet ELISA kit (R&D Systems; Wiesbaden, Nordenstadt, Germany) following the manufacturer’s instructions, with a plasma dilution of 1:5000 for analysis. The human IL-6 DuoSet ELISA kit from R&D Systems was used for IL-6 analysis, and plasma was 2-fold diluted.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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