2.8.2. Cell viability assay.

Mahalanobis Taguchi system (MTS) tests were done on laryngocarcinoma cells using a Promega Kit (Madison, WI) according to the manufacturer’s instructions. In brief, 3000 Hep-2 cells were seeded onto 96-well plates at 100 L/well and grown under various treatment settings. Following the required time, 10 L of MTS solution was added to 90 L of RPMI-1640 each well, and the plates were incubated for 30 minutes. Following that, the absorbance of each well was measured at 490 nm using a microplate reader (Bio-Rad, USA).