Liver tissue samples were collected from control subjects (n = 4) and NASH patients with fibrosis scores F0 (n = 4), F2 (n = 4), and F3 (n = 4), followed by tissue fixation, and stained with Hematoxylin and Eosin (H&E). BMP8B immunohistochemistry was performed on tissue sections using primary monoclonal rabbit antibodies (CSB-PA050097) and the assay was performed by following the standard procedure. The staining was observed under a microscope and images were taken at 40X.
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