Mice were placed in cages (37 cm × 28 cm × 13 cm) covered with a wire on the top so they could move freely and were not stressed. The mice were acclimatized for one week under reversed light-dark conditions (12 h light and 12 h dark) at 23–26 °C and 40–60% humidity. The mice received a standard feed (15% BW/day) and drinking water ad libitum.
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