2.1. Material and reagents

JY Jingchun Yang
SD Shuaiyi Dong
XZ Xu Zhou
WZ Wen Zhang
YG Yunzhu Gu
LZ Lixue Zheng
GY Guihong Yang
JW Jing Wang
YZ Yang Zhang
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The waste Z. mioga leaves were collected from a Z. mioga planting zone on 16 September 2022 (Xuanen, Hubei, China). Nanjing Jiancheng Bioengineering Institute (Jiangsu, China) provided the commercial kits for determining anticoagulant biomarkers, and Siemens Healthcare Diagnostics Products GmbH (Marburg, Germany) offered the standard human plasma. Other solvents and reagents with analytical grade were obtained from the local reagent companies. The experimental flow chart of this study was depicted in Fig. 1.

The experimental flow chart of this work.

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