The surface morphology of nanofibers containing nano-encapsulated genistin before and after cell culture was monitored by scanning electron microscopy (SEM). Cell-seeded scaffolds were washed with PBS and fixed with 2.5% glutaraldehyde for 40 min. Then the scaffolds were dehydrated with increasing alcohol concentration. Finally, the samples were allowed to dry at room temperature (RT) and sprayed with gold. The acceleration voltage of the scanning electron microscope was 24 kV.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.