2.2.3. Hematoxylin and eosin staining

Formalin‐fixed brain specimens were dehydrated in a series of diluted ethanol solutions for 10 min each, cleared with xylene, and embedded in paraffin. Coronal sections were trimmed to a thickness of 5 μm and mounted on slides, which were heated to adhere to the tissue. Hematoxylin and eosin (H&E) staining was performed for 3 min, followed by dehydration with alcohol. The sections were cleared with xylene and mounted with neutral resin. Pathological changes were observed under a light microscope and assessed based on previous literature (Chen et al., ²⁰¹⁹).