Animals:

The following lines: Cspg4-creER⁷¹ (JAX #008538); Ai9⁷² (JAX #007909); PhAM⁷³ (JAX #018385) were bred to generate the Cspg4-creER: Ai9: PhAM triple transgenic mice. This mouse line allows for conditional visualization of mitochondria (mitoDendra2 labeled) and cytoplasm (tdTomato labeled) in cells expressing the chondroitin sulfate proteoglycan 4 (Cspg4, also known as NG2) and their progeny. A single dose of tamoxifen (0.4–0.8 mg) was intraperitoneally injected at P25. This produced sparse dual or single recombination providing double and single tdTomato-only or mito-Dendra2-only labeled cells. Both male and female mice aged P30-P651 were used for the experiments. All animals were housed in a temperature and humidity-controlled vivarium in a 12h light/dark cycle with ad libitum access to food and water. All the animal procedures were approved by the Institutional Animal Care and Use Committee at Dartmouth College.