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MW Maolin Wang
CG Chenming Gu
YY Yifu Yang
LC Liang Chen
KC Kaixian Chen
JD Jun Du
HW Huali Wu
YL Yiming Li
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Tandem mass spectra were processed by PEAKS Studio version 10.6 (Bioinformatics Solutions Inc., Waterloo, Canada). PEAKS DB was set up to search the database of uniport-Mus musculus (version 201907, 22290 entries) assuming trypsin as the digestion enzyme. PEAKS DB were searched with a fragment ion mass tolerance of 0.02 Da and a parent ion tolerance of 10 ppm. Carbamidomethylation (C) and TMT-10 plex were specified as the fixed modification. Oxidation (M) and Acetylation (Protein N-term) were specified as the variable modifications. Peptides were filtered by 1% FDR and proteins were filtered by 1 unique peptide. Reporter ions were used to calculate the quantification ratio between samples. Normalization was calculated from the total intensity of all labels in all quantifiable peptides. After t-test, different expressed proteins were selected if their p < 0.05 and |fold change| >1.5.

Copyright and License information:  ©2023 Wang, Gu, Yang, Chen, Chen, Du, Wu and Li.
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