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Cryopulverization and DNA/RNA extraction
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Cryopulverization was performed using Covaris CP02 CryoPrep Pulverizer and RNA extraction was performed as described by Zhou and colleagues (30). Genomic DNA was extracted with the QIAamp DNA Mini Kit (Qiagen, 51304). Total RNA was run on TapeStation (Agilent Technologies) and samples with RNA integrity number equivalent (RINe) greater than 7 were subjected to RNA sequencing.
Copyright and License information: ©2023 The Authors; Published by the American Association for Cancer Research
This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
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