2.2. Primers and probe of the IAV HF-LAMP assay

Five sets of IAV RT-LAMP primers have been described previously (Table S1) [[17], [18], [19], [20], [21]]. The primer set-2 was used to develop the IAV HF-LAMP assay. The LF of the Set-2 was designed as an HFman probe by labeling a Cy5 fluorophore and a BHQ2 quencher group at its 3′- and 5′-ends, respectively. The primers and HFman probe were synthesized by Sangon Biotech (Shanghai) Co., Ltd,.