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mRNA expression analyses

YM Yingying Meng
WZ Wenwen Zhang
ZW Zhaoming Wang
FY Feng Yuan
SG Sandui Guo
HL Hao Lin
LN Lifang Niu
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Total RNA was extracted from the dissected leaves at six-leave stage using TRIzol™ Reagent (Invitrogen, 15596018CN, USA) according to the manufacturer’s instructions. Approximately 2 μg of the total RNA was used as a template to synthesize cDNA with EX RT Kit (gDNA remover) (ZOMANBIO, ZR108-2, China). Quantitative RT-PCR was performed as previously described (Meng et al. 2019), using Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02, China), with three biological and three technical replicates. Gene expression was normalized using the expression of the housekeeping gene ACC1 (Alexander et al. 2007). All primers used are listed in Table S1.

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