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PBMCs were purified from blood samples by density centrifugation using Ficoll-Paque (Eurobio, Les Ulis, France), washed twice in Hank’s Balanced Salt Solution (LONZA, Basel, Switzerland), and then resuspended at a concentration of 2 × 106 cells per mL in Roswell Park Memorial Institute (RPMI)-1640 (LONZA, Basel, Switzerland) with 10% fetal bovine serum, 2 mM glutamine, 1× minimum essential medium nonessential AA, 1 mM Na-pyruvate, 50 µM 2-betamercaptophenol, and 40 µg/mL gentamicin (Sigma, MO, USA).
Copyright and License information: ©2023 Bchiri et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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